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Methacrylate resin based techniques in histopathology for soft and hard specimens

When disease intervenes, diagnostic processes may require microscopy services for an accurate clinical evaluation of underlying histopathology. Conventional methodologies become rapidly outdated when new advances in surgical procedures are made. In order to remain at the forefront of providing radical histological approaches researchers have to accommodate new techniques in line with modern clinical needs to visualise components of mineralised and soft tissues with and/or without interventional devices. At UCLan, we are specialists in this very skilful technique.

Project Lead

Dr Sim K. Singhrao

Timeline

2012-

Impact

Potential diagnostic aid

Public Outputs

Singhrao SK, Müller CT, Gilbert SJ, Duance V, Archer CW. An immunofluorescence method for post-embedded tissue in acrylic resin Technovit 9100 New® using fluorescein isothiocyanate secondary detection marker. Microsc Res Tech 2009; 72(7): 501-506.

Singhrao SK and Nair-Roberts RG. In-situ hybridisation and immunofluorescence on resin embedded tissue to identify the components of Nissl substance. Microsc Res Tech 2010; 73(5):555-9.

Singhrao SK, Sloan AJ, Smith EL Archer CW. Technical advances in the sectioning of dental tissue and of on-section cross-linked collagen detection in mineralised teeth. Microsc Res Tech 2010; 73(8):741-5..

Singhrao SK, Nicholson K, Crean S. Informed choices for challenging specimens when choosing Methacrylate resins for histology. Microsc Res Tech 2012; 75(5):576-85.

McCarthy HE, Bara JJ, Brakspear K, Singhrao SK, Archer CW. The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse. Vet J. 2012; 192(3):345-51.