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    Membranes, Membrane Proteins and Peptides

Membranes, Membrane Proteins and Peptides research group

The scope of research within this grouping in the School of Pharmacy and Biomedical Sciences is broad, ranging from fundamental investigations of how molecules (including more complex molecules such as antimicrobial peptides) interact with biological membranes (Adams, Dennison, Phillips-Jones, Shaw, Snape) and with membrane proteins (Alder, Phillips-Jones), the structures and functions of membrane proteins including ligand and inhibitor binding studies (Phillips-Jones), to membrane studies in relation to nanoparticle design for drug delivery, including the use of polymeric and lipidic systems (Singh, Wan). The group is focused on fundamental studies of and therapies for a range of diseases including cancer, dermatological conditions and infectious diseases. Examples include the cellular causes of neurodegenerative disorders such as Alzheimer’s disease (Jones), the preparation of benzamidobenzoic acid derivatives and analogs for use as retinoic acid receptor α agonists (Adams), the differentiation of embryonical carcinoma cells (Shaw), mast cell structure, function and interactions (Passante) and the development of novel therapies for the treatment of Diabetes Mellitus, with a specific emphasis on the potential use of glucagon receptor antagonists (McShane).

The Group operates a 'low-wall' principle, to promote strong interactions with other groups within the School, thereby encouraging cross-disciplinary research and integrative approaches to major pharmaceutic and biomedical questions. The group is housed within the University’s Biomedical Research Facility and the adjacent Maudland Building, providing a stimulating research environment for research and research training at the highest levels.

The Group uses a range of cutting-edge biomolecular and biophysical methods and holds strong national and international collaborations and links with a number of external research facilities and institutions, including the Helmholtz Zentrum Beriin für Materialien und Energie (HZB) (Berlin), the Centro de Investigação Interdisciplinar Egas Moniz (Portugal), the West China School of Pharmacy (Sichuan University, China), Kyushu University (Japan), the Membrane Protein Laboratory for X-ray crystallography and Beamline B23 at the Diamond Light Source Ltd (Oxon).

For more information contact MPhillips-Jones@uclan.ac.uk

Expertise and Subject Areas

Professor Kamalinder Singh – Nanomedicine, drug delivery systems. Kamalinder has extensive research experience in the field of pharmaceutical nanoscience and nanoparticle design for drug delivery. Research efforts of her group focus on drug delivery systems, nanoemulsions, lipid nanostructures, protein based nanoparticles, nanosuspensions, microspheres, long acting parenterals as well as surface functionalization. These drug delivery methods are being applied for overcoming biological barriers, targeting and treatment in variety of diseases including cancer, dermatological conditions, infectious and parasitic diseases including malaria. Various nanotechnologies have been used alone or in combination to prepare mixed nano-systems for delivery of single drug or co-delivery of multi agents. She has also applied these technologies to herbal drugs and actively involved in development of novel herbal drug delivery systems. A major challenge in the drug delivery field is to enhance transport of therapeutics across biological barriers such as the blood brain barrier (BBB), the small intestine, nasal, skin and the mouth mucosa

Dr David R. Adams - computer-aided design and high throughput synthetic techniques. His research interests are concerned with the medicinal chemistry of CNS disorders including schizophrenia and Alzheimer’s disease and of parasitic diseases including malaria and Trypanosoma cruzi.

Dr Jane Alder – pharmacology and efflux and uptake transporters. Jane’s research interests include membrane efflux and uptake transporters, following collaborative research which identified the role and importance of transporter-enzyme interplay in determining drug/inhibitor concentrations and inhibitory potency in hepatic microsomes compared with hepatocytes. She and her collaborators showed that, due at least in part to transporter functions, drug-drug interaction predictions are not always intuitive.

Dr Sarah R. Dennison – biophysics and antimicrobial peptides. Sarah is a research associate interested in the characterisation of the biological activities of amphiphilic molecules, particularly antimicrobial and anticancer peptides. She has published over 50 papers as well as a book titled Antimicrobial peptides (Phoenix, D. A., Dennison, S. R., and Harris, F. eds.), Wiley, Germany. Her laboratory is currently pursuing a number of questions concerning the mechanism of action of antimicrobial peptides (AMPs) and anticancer peptides (ACPs). The research is divided into two areas involving theoretical and practical based research. The theoretical approach investigates the structure / function relationships of AMPs and ACPs and has established that architectural features of α-ACPs such as amphiphilicity levels and hydrophobic arc size are of major importance to the ability of these peptides to invade microbial and/or cancer cell membranes cells. The practical side of the research investigates peptide lipid interactions using a variety of techniques. The main technique involves the use of Langmuir Blodgett troughs, which is a powerful technique used for studying peptide interactions with lipid membranes. Langmuir Blodgett troughs are used to investigate the surface activity, peptide structure at an air/water interface, the ability of the peptide to interact with various lipids and lipid systems mimetic of bacterial /tumour membranes. Circular dichroism is used to investigate the structure of the peptide in the presence of lipid systems mimetic of bacterial/tumour membranes. Fluorescence spectroscopy is technique also used to investigate the mechanism of peptide membrane interaction providing information of peptide vesicle leakage and peptide binding.

Dr Vicky C. Jones – Cell biology and molecular biology. Vicky has experience in the application of molecular biology techniques to cell biology. For example, she has developed new lentivirally-delivered fluorescent reporter systems to visualise the structure of the endoplasmic reticulum (ER) in cultured neurons and in brain slices. Using these reporters Vicky provided the first direct evidence of a single, continuous ER lumen extending throughout the cell both in neurons and in astrocytes. Most recently, she has applied her molecular biology expertise in collaborative research aimed at identifying novel inhibitors of eukaryotic cells such as infectious Saccharomyces cerevisiae.

A HeLa cell expressing the early endosomal pathway marker, Rab-5 GFP. Psudocoloured for fluorescence intensity, with edges intensified. Blue represents the DAPI-stained nucleus (Vicky Jones).

Dr Laura McShane - Diabetes and glucagon receptor antagonists. Laura has interests in the potential use of incretin mimetics and other peptide analogues for the treatment of diabetes mellitus. Her research focuses on the effects of these hormones in vitro (using clonal pancreatic beta cell lines) and in vivo. She is particularly interested in the role of glucagon-like peptides (including GLP-1, glucagon and oxyntomodulin) in the pathogensis and prevention of disease. In collaboration with Dr Mary Phillips-Jones, she is also interested in how glycopeptide antibiotics exert their inhibitory effects on bacteria through interactions with bacterial membrane receptors which in turn then relay signal information from the membrane to intracellular signal transduction systems.

Dr Egle Passante – Immunopharmacology, chemistry and pharmaceutical technologies. Egle has a wealth of experience in synthetic chemistry, flow cytometry, identification and classification of drug interactions by HTS screening, Western blotting and tissue culture techniques, with the latter particularly applied to research studies of mast cell physiology. At UCLan, she has initiated her independent research on how mast cells regulate their survival post-sensitisation. She is also working on bacterial-mast cell interactions in collaboration with Dr Mary Phillips-Jones.

Dr Mary K. Phillips-Jones – Structure and function of bacterial signal transduction pathways, two-component pathways, membrane sensory proteins. Mary’s research interests lie principally with how bacteria sense and respond to environmental change, focusing particularly on the structure and function of bacterial signal transduction pathways (mainly two-component signal transduction systems) and regulators of bacterial gene expression. Research includes investigations of intact sensory proteins that are usually located in the bacterial membrane and are responsible for sensing environmental stimuli and stresses, and also their partner cytosolic regulators that effect appropriate adaptive responses (usually by binding to specific promoters and thereby changing gene expression). The principal systems/regulators (and their associated pathways) under investigation are those found in a range of pathogenic bacteria associated with hospital-acquired infections. The main approaches and tools of the research are: biophysical methods to investigate ligand -protein interactions of the signal transduction proteins involved, including the intact membrane sensor kinases; elucidation of the three-dimensional structures with a view to structure-based drug design; and phosphorylation-based activity assays to screen and identify candidate signalling ligands and inhibitors.

Dr Lisa Shaw – Aptamers which have antimicrobial activity by binding to membrane and cell wall proteins. Other research areas include cell biology and gene expression. Current research involves using Aptamers in in vitro and in vivo applications. Another area of interest lies with miRNAs and how these may regulate cell processes in disease.

Dr Tim J. Snape – Synthetic chemistry. Tim’s research interests in the area of membranes focus on being able to exploit the known tendency for certain molecules to adopt defined shapes and thus, once suitably adorned, interact with phospholipid monolayers. He and his colleagues have shown that a strong link exists between conformation and the mechanism of membrane binding, and that this effect may be driven by amphiphilicity. They have also demonstrated that such molecules can have weak→potent activity against membranes composed of lipids from Escherichia coli, Staphylococcus aureus and membranes which mimic cancer membranes, and that activity is dependent upon both the amphiphilicity and length of the molecule.

Dr Ka-Wai Wan - Ka-Wai’s research interests lie in the field of nanoparticulate drug delivery systems including the use of polymeric and lipidic systems, for targeted therapy and delivery of small and macromolecules such as genes and proteins. In addition, exploitation of these nanoparticulate drug delivery systems to help enhance transdermal delivery is also being investigated. She is particularly interested in the applications and biological fate of these targeted delivery systems for the treatment of various diseases such as cancer and inflammatory conditions. An understanding of the membrane interaction with these nanosystems is fundamental to the design of these drug delivery systems with improved clinical outcomes. Current research projects involve targeting to the tumour endothelium using integrin-targeted nanoparticles as a means of anti-angiogenic or vascular targeting for the treatment of cancer, and nanoparticulate drug delivery system for enhanced localisation and delivery of anti-infectives through the skin. The membrane-permeabilising effects and uptake of these drug delivery systems are being explored in these studies.

Impact

See publications and outputs. The group is also actively engaged in exploitation of its research and in dissemination of research findings to the general public through events such as the Lancashire Science Festival and Scientific Open days. The list below are recent examples of public engagement from Dr Phillips-Jones’s group:

Diamond Light Source Ltd Open Day: 6th April 2013: Demonstration of use of sucrose density gradient centrifugation and other methods to purify bacterial membrane proteins: ‘Techniques for preparing purified FsrC membrane sensor protein for synchrotron radiation circular dichroism (SRCD) spectroscopy measurements of ligand binding at Beamline B23’ by Mary K. Phillips-Jones, Simon G. Patching, Shalini Edara, Jiro Nakayama, Rohanah Hussain and Giuliano Siligardi.

Diamond Light Source Ltd Open Day: 6th April 2013: Demonstration of inhibitor studies using membrane proteins: ‘Applications of synchrotron radiation circular dichroism (SRCD) for studies of ligand and inhibitor interactions with membrane protein targets: investigations of the FsrC quorum membrane sensor kinase’. Simon G. Patching, Shalini Edara, Jiro Nakayama, Rohanah Hussain, Giuliano Siligardi & Mary Phillips-Jones.

Diamond News 15th February 2013: Diamond News ‘A tale of two beamlines’ Spring 2012: Highlighting the first research report on the successful use of SRCD spectroscopy to obtain quantitative ligand binding data for any membrane protein (Patching, S.G., Edara, S., Ma, P. Nakayama, J., Hussain, R., Siligardi, G. & Phillips-Jones, M.K. (2012) Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism. Biochim. Biophys. Acta Biomembr. 1818, 1595-1602).

Phillips-Jones, M.K., Patching, S.G., Edara, S., Nakayama, J., Hussain, R., & Siligardi, G. (2013). Investigation of the first step of a virulence regulatory pathway in a bacterial hospital-acquired infection agent. Diamond Light Source Annual Review 2012/13. pp30-31.

Publications and Outputs

Original research publications in the past three years:

2014 (compiled March 2014)

Phillips-Jones, M.K. (2014) Structural and biophysical characterisation of membrane protein-ligand binding. Biochim. Biophys. Acta Biomembr. 1838 Issue 1 Part A 1-2

Siligardi, G., Hussain, R., Patching, S.G. & Phillips-Jones, M.K. (2014). Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy. Biochim. Biophys. Acta Biomembr. 1838: 34-42

Okoh, O. A., Bisby, R. H.; Lawrence, C.L. and Rolph, C.E. (2014) Promising near-infrared non-targeted probes: benzothiazole heptamethine cyanine dyes. Journal of Sulfur Chemistry 35: 42-56.

Keck CM, Anantaworasakul P, Patel M, Okonogi S, Singh KK, Roessner D, Scherrers R, Schwabe K, Rimpler C, Müller RH A new concept for the treatment of atopic dermatitis: silver-nanolipid complex (sNLC), International Journal of Pharmaceutics. 462(1-2):44-51 (2014)

Rajani B. Athawale , Darshana S. Jain , Kamlinder K. Singh, Rajiv P. Gude, Etoposide loaded solid lipid nanoparticles for curtailing B16F10 melanoma colonization in lung, Biomedicine & Pharmacotherapy, 68 (2), 231-240, (2014).

Tumilson. C.A., Lea. R.W, Alder. J.E., Shaw. L., Circulating MicroRNA Biomarkers for Glioma and Predicting Response to Therapy, In press.

Prabhu, S., Harris, F., Lea, R.W. and Snape, T.J. (2014) A review of small molecule clinical trial candidates for the treatment of glioma. Drug Discovery Today, In press.

Ameen, D. and Snape, T.J. (2014) Developing the scope of O→C aryl migrations; exploring amide substrates as potential precursors for new asymmetric reactions. Eur. J. Org. Chem., 2014, In press.

Lal, S. and Snape, T.J. (2014) Towards a sustainable synthesis of aniline-derived amides using an indirect chemoenzymatic process: challenges and successes. RSC Advances, 2014, 4 (4), 1609 - 1615.

2013

Harris, F., Dennison, S. R., Singh, J., and Phoenix, D. A. (2013) On the selectivity and efficacy of defense peptides with respect to cancer cells, Medicinal Research Reviews 33, 190-234.

Mura, M., Dennison, S.R., Zvelindovsky,, A.V., and Phoenix, D.A. 2013. Aurein 2.3 functionality is supported by oblique orientated α-helical formation Biochimica et Biophysica Acta (BBA) - Biomembranes, 1828(2) 586-594

Prabhu, S., Dennison, S. R., Lea, B., Snape, T.J., Nicholl, I.D., Harris, F. 2013. Anionic Antimicrobial and Anticancer Peptides from Plants. Critical Reviews in Plant Sciences (5): 303-320

Dennison, S. R., Phoenix, D. A., and Snape, T. J. (2013) Synthetic oligoureas of metaphenylenediamine mimic host defence peptides in their antimicrobial behaviour, Bioorganic & Medicinal Chemistry Letters 23, 2518-2521.

Oliveira, L. M., Gomes, R. A., Yang, D., Dennison, S. R., Familia, C., Lages, A., Coelho, A. V., Murphy, R. M., Phoenix, D. A., and Quintas, A. (2012) Insights into the molecular mechanism of protein native-like aggregation upon glycation, Biochimica et Biophysica Acta 1834, 1010-1022.

Dennison, S. R., Harris, F., Morton, L. H., and Phoenix, D. A. (2013) Antimicrobial activity of aurein 2.5 against yeasts, FEMS Microbiology Letters. 346, 140-145

Dennison, S.R., Harris, F., Mura, M., Zvelindovsky,, A.V., and Phoenix, D.A. (2013). A novel form of bacterial resistance to the action of eukaryotic host defense peptides, the use of a lipid receptor, Biochemistry. 52, 6021-6029.

Tyler AR, Okoh AO, Lawrence CL, Jones VC, Moffatt C, Smith RB (2013) N-Alkylated 2,3,3-trimethylindolenines and 2-methylbenzothiazoles. Potential lead compounds in the fight against Saccharomyces cerevisiae infections. Eur J Med Chem, 64C:222-227.

Phillips-Jones, M.K., Patching, S.G., Edara, S., Nakayama, J., Hussain, R., & Siligardi, G. (2013). Investigation of the first step of a virulence regulatory pathway in a bacterial hospital-acquired infection agent. Diamond Light Source Annual Review 2012/13. pp30-31.

Phillips-Jones, M.K., Patching, S.G., Edara, S., Nakayama, J., Hussain, R., & Siligardi, G. (2013) Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide siamycin I revealed through synchrotron radiation circular dichroism. Phys. Chem. Chem. Phys. 15, 444-447.

Cooney, Austin John, Shaw, Lisa, Sneddon, Sharon F., Zeef, Leo, Kimber, Susan J. and Brison, Daniel R. (2013) Global Gene Expression Profiling of Individual Human Oocytes and Embryos Demonstrates Heterogeneity in Early Development. PLoS ONE, 8 (5). e64192. ISSN 1932-6203.

Patel, M., Singh, K.K. and Souto, E.B., Advances in Brain Drug Targeting and Delivery: Limitations and Challenges of Solid Lipid Nanoparticles, Expert Opinion on Drug Delivery, July 2013, Vol. 10, No. 7 : Pages i-ii

Pople, P.V. and Singh, K. (2013) Development and evaluation of colloidal modified nanolipid carrier: Application to topical delivery of tacrolimus, Part II – In vivo assessment, drug targeting, efficacy, and safety in treatment for atopic dermatitis. European Journal of Pharmaceutics and Biopharmaceutics, 84 (1). pp. 72-83. ISSN 09396411

Hamad Ameen, D.M. and Snape, T.J. (2013) Chiral 1,1-diaryl compounds as important pharmacophores. Med. Chem. Commun., 4: 893 - 907.

Prabhu, S., Akbar, Z., Harris, F., Karakoula, K., Lea, R.W., Rowther, F., Warr, T. and Snape, T.J. (2013) Preliminary biological evaluation and mechanism of action studies of selected 2-arylindoles against glioblastoma. Bioorg. Med. Chem. 21: 1918-1924.

Judd, A. M., Scurr, D. J., Heylings, J. R., Wan, K-W., Moss, G. P. (2013). Distribution and Visualisation of Chlorhexidine Within the Skin Using ToF-SIMS: A Potential Platform for the Design of More Efficacious Skin Antiseptic Formulations. Pharmaceutical Research, 30(7): 1896-1905

Hussein, I., Stasik, A., Wan, K-W., Ahmed, W., Phoenix, D., Ahmed, E., Elhissi, A. (2013). Manufacture and Characterization of Asiatic Acid Non-Ionic Surfactant Nanocapsules. Journal of Manufacturing Technology Research, 5(1-2 )

Kadam, A., Najlah, M., Wan, K-W., Ahmed, W., Phoenix, D., Taylor, K., Elhissi, A. (2013). Stability of Parenteral Nanoemulsions Loaded with Paclitaxel: The Influence of Lipid Phase Composition, Drug Concentration and Storage Temperature. Pharmaceutical Development and Technology, 19(8):999-1004

2012

Ashmore J, Pickett J, Alder J, Marks R, Thorniley M. (2012) Whole body oxygen delivery and consumption during cardiopulmonary bypass surgery. Adv. Exp. Med. Biol. 737:229-34.

Dennison S.R, Akbar Z, Phoenix D.A, Snape T.J. 2012. Interactions between suitably functionalised conformationally distinct benzanilides and phospholipid monolayers. Soft Matter. 8: 3258-3264

Harris, F, Dennison, S.R. and Phoenix, D.A. 2012. Aberrant action of amyloidogenic host defense peptides: a new paradigm to investigate neurodegenerative disorders? The FASEB Journal. 2012 May;26(5):1776-81

Dennison, S. R. Morton LHG and Phoenix D.A. 2012. Effect of amidation on antimicrobial peptide aurein 2.5 from Australian southern bell frogs. Protein and Peptide Letters. 19(6):586-91.

Dennison, S. R. Morton LHG and Phoenix D.A 2012. Role of molecular architecture on the relative efficacy of aurein 2.5 and modelin 5

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1818(9) 2094-2102

Dennison SR, Phoenix AJ, Phoenix DA 2012 Effect of salt on the interaction of Hal18 with lipid membranes. Eur Biophys J. 41(9):769-76.

Dennison SR, Snape TJ, Phoenix DA.2012. Thermodynamic interactions of a cis and trans benzanilide with Escherichia coli bacterial membranes.Eur Biophys J. 2012 Aug;41(8):687-93.

Patching, S.G., Edara, S., Ma, P. Nakayama, J., Hussain, R., Siligardi, G. & Phillips-Jones, M.K. (2012) Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism. Biochim. Biophys. Acta Biomembr. 1818, 1595-1602.

Patching, S.G., Middleton, D.A., Kalverda, A., Gowdy, J., Baldwin, S.A., Phillips-Jones, M.K., Levitt, M.H., Homans, S.W. & Henderson, P.J.F. (2012) NMR methods for structure-function investigation of membrane proteins and their ligands. J. Labell.Comp. Radiopharm. 55, 132-133.

Laussmann, M. A., Passante, E., Hellwig, C. T., Tomiczek, B., Flanagan, L., Prehn, J. H. M., Huber, H. J. and Rehm, M. (2012) Proteasome Inhibition Can Impair Caspase-8 Activation upon Submaximal Stimulation of Apoptotic Tumor Necrosis Factor-related Apoptosis Inducing Ligand (TRAIL) Signaling. Journal of Biological Chemistry, 287 (18). pp. 14402-14411. ISSN 0021-9258

Blakey, R. T.; Mason, A.; Al-Shamma'a, A. I.; et al. (Rolph, C.E.) (2012) Utilisation of a Resonant Structure to Differentiate Lipomyces Yeast Cultures based upon Lipid Content. Conference: 6th International Conference on Sensing Technology (ICST) Location: Kolkata, INDIA Date: DEC 18-21, 2012. 2012 SIXTH INTERNATIONAL CONFERENCE ON SENSING TECHNOLOGY (ICST) Book Series: International Conference on Sensing Technology Pages: 694-697.

Shaw, L., Sneddon, S. F., Brison, D. R. and Kimber, S. J. (2012) Comparison of gene expression in fresh and frozen-thawed human preimplantation embryos. Reproduction, 144 (5). pp. 569-582. ISSN 1470-1626.

Pople P.V., Singh, K. K. Targeting tacrolimus to deeper layers of skin with improved safety for treatment of atopic dermatitis - Part II: In vivo assessment of dermatopharmacokinetics, biodistribution and efficacy. International Journal of Pharmaceutics, 434: 70– 79, (2012)

Singh K.K., Parmar, S.,Tatke, P.A., HerbOshield™ vaginal gel in rats, Contraception, 85, (1), 122-127, (2012)
Shegokar R., Singh, K. K., Nevirapine nanosuspensions: stability, plasma compatibility and sterilization Journal of Pharmaceutical Investigation, 42:257–269 (2012)

2011

Lappin, Graham, Shishikura, Yoko, Jochemsen, Roeline, Weaver, Richard John, Gesson, Charlotte, Houston, J. Brian, Oosterhuis, Berend, Bjerrum, Ole J., Grynkiewicz, Grzegorz, Alder, Jane, Rowland, Malcolm and Garner, Colin (2011) Comparative pharmacokinetics between a microdose and therapeutic dose for clarithromycin, sumatriptan, propafenone, paracetamol (acetaminophen), and phenobarbital in human volunteers. Europ. J. Pharmaceut. Sci. 43 (3). pp. 141-150.

Newman, William G, Payne, Katherine, Tricker, Karen, Roberts, Stephen A, Fargher, Emily, Pushpakom, Sudeep, Alder, Jane, Sidgwick, Gary P, Payne, Debbie, Elliott, Rachel A, Heise, Marco, Elles, Robert, Ramsden, Simon C, Andrews, Julie, Houston, J Brian, Qasim, Faeiza, Shaffer, Jon, Griffiths, Christopher EM, Ray, David W, Bruce, Ian and Ollier, William ER (2011) A pragmatic randomized controlled trial of thiopurine methyltransferase genotyping prior to azathioprine treatment: the TARGET study. Pharmacogenom. 12 (6). pp. 815-826.

Dennison, S.R. and Phoenix, D.A. 2011. Influence of C-terminal amidation on efficacy of modelin-5. Biochemistry. 50(9) 1514-1523

Harris, F, Dennison, S.R. and Phoenix, D.A. 2011. Anionic antimicrobial peptides from eukaryotic organisms and their mechanisms of action. Current Chemical Biology. 5(2) 142-153

Dennison, S.R. and Phoenix, D.A. 2011. Effect of cholesterol on the membrane interaction of modelin-5 isoforms. Biochemistry. 50(50) 10898-10909.

Dennison, S.R. and Phoenix, D.A. 2011. Effect of cholesterol on the membrane interaction of modelin-5 isoforms. Biochemistry. 50(50) 10898-10909.

Laussmann, M A, Passante, E., Düssmann, H, Rauen, J A, Würstle, M L, Delgado, M E, Devocelle, M, Prehn, J H M and Rehm, M (2011) Proteasome inhibition can induce an autophagy-dependent apical activation of caspase-8. Cell Death and Differentiation, 18 (10). pp. 1584-1597. ISSN 1350-9047

Ma, P., Yuille, H.M., Nishiguchi, K., Nakayama, J. & Phillips-Jones, M.K. (2011) Anti-HIV siamycin I directly inhibits autophosphorylation activity of the bacterial FsrC quorum sensor and other ATP-dependent enzyme activities. FEBS Letters 585, 2660-2664.

Blakey, R. T.; Mason, A.; Rolph, C. E.; et al. (2011) Lipid droplet detection by the cavity perturbation method. Edited by: Kyriacou, PA; O'Riordan, A; McConnell, G. Conference: 16th Conference in the Biennial Sensors and their Applications Location: Univ Coll Cork, Tyndall Natl Inst, Cork, IRELAND Date: SEP 12-14, 2011 SENSORS & THEIR APPLICATIONS XVI Book Series: Journal of Physics Conference Series Volume: 307 Article Number: 012021 Published: 2011

Pople P.V., Singh, K. K., Development and evaluation of colloidal modified nanolipid carrier: Application to topical delivery of tacrolimus. Eur. J. Pharm. Biopharm. 79, 82–94 (2011)

Shegokar, R., Jansch M, Singh, K.K., Müller, R.H. In vitro protein adsorption studies on nevirapine nanosuspensions for HIV chemotherapy, Nanomedicine : Nanotechnology, Biology and Medicine, 7, 333–340, (2011)

Shegokar, R.,Singh, K.K., Surface modified nevirapine nanosuspension for viral reservoir targeting : in vitro in vivo evaluation International Journal of Pharmaceutics 2011 Oct 1. [Epub ahead of print] DOI information: 10.1016/j.ijpharm.2011.09.041

Singh, K. K. Pople P.V., Safer than safe: lipid nanoparticulate encapsulation of tacrolimus with enhanced targeting and improved safety for atopic dermatitis, J. Biomed. Nanotechnology, 2011, 7(1):40-1

Shegokar R., Singh, K. K., Mueller, R.H., Production & Stability of Stavudine Solid Lipid Nanoparticles-from Lab to Industrial Scale, International Journal of Pharmaceutics, : 416 (2), 461-470 (2011)

Shegokar, R.,Singh, K.K., Müller, R.H., 7, 333–340, (2011) Nevirapine nanosuspension: comparative investigation of production methods, Nanotechnology Development, 1: e4, 16-22 (2011)

Shegokar R, Singh KK: Nevirapine nanosuspensions for HIV reservoir targeting, Die Pharmazie , 66, 408–415, (2011)

Shegokar R., Singh, K. K., Stavudine Entrapped Lipid Nanoparticles for Targeting Lymphatic HIV Reservoirs, Die Pharmazie, 66, 264-271 (2011)

Shegokar R.., Singh, K. K., Conversion of stavudine lipid nanoparticles into dry powder, International Journal of Pharma and Bio Sciences, 2(1),443 (2011)

Snape, T.J., Karakoula, A., Rowther, F. and Warr, T.J. (2011), Exploiting conformationally restricted ureas as biologically active C=C double bond analogues against GBM cells in vitro Neuro. Oncol., 2011, 13 (suppl 2): ii1-ii14.

Prabhu, S., Harris, F., Lea, R.W. and Snape, T.J. (2011) Towards establishing the effects and mechanism of action of a series of indoles in an in vitro chemosensitivity system for glioma treatment . Neuro. Oncol., 2011, 13 (suppl 2): ii1-ii14.

Members

Professor Kamalinder Singh

Dr Jane Alder

Dr Sarah R. Dennison

Dr Vicky C. Jones

Dr Egle Passante

Dr Milos Petrovic

Dr Mary K. Phillips-Jones

Dr Lisa Shaw

Dr Tim J. Snape

Dr Ka-Wai Wan

Visiting Members

Professor Alok Dhawan (Director of Institute of Life Sciences, Ahmedabad University, Gujarat and Senior Principal Scientist and Area Coordinator, Nanomaterial Toxicology Group CSIR-Indian Institute of Toxicology Research, Lucknow, India).

Professor Gert Storm (Controlled Drug Delivery group, University of Twente, The Netherlands)

 

Links

Nanotechnology

www.pharmweb.net

Related Research Groups

The Group uses a range of cutting-edge biomolecular and biophysical methods and holds strong national and international collaborations and links with a number of external research facilities and institutions, including:

 

  • the Helmholtz Zentrum Beriin für Materialien und Energie (HZB) (Berlin);
  • the Centro de Investigação Interdisciplinar Egas Moniz (Portugal);
  • the West China School of Pharmacy (Sichuan University, China);
  • Kyushu University (Japan);
  • the Membrane Protein Laboratory at the Diamond Light Source Ltd.;
  • the Diamond Light Source Ltd, Harwell Innovation Campus, Didcot, Oxfordshire.

 

Cross collaboration between research groups in the School of Pharmacy & Biomedical Sciences are also in place:

Clinical Practice Group

Neuronal and Tissue Dysfunction

Institute of Nanotechnology and Bioengineering

Pharmaceutics – Drug Design, Analysis & Delivery

Brain Tumour North West