Streptozotocin-induced diabetic rats: cerebrocortical insulin, glucose and ATP levels and behaviour, with relevance to the switching of modes of neuronal exocytosis

Changes in exocytotic mode in the glutamatergic neurons of diabetic rats may be related to changes in cognition and behaviour. The increase in stress hormones associated with STZ diabetes may have an effect on both the mode of exocytosis and the behaviour. The aims of the research:

  1. To study the bioenergetics of nerve terminals in control and STZ-induced diabetic rats.
  2. To establish the effects of STZ-induced diabetes on brain insulin levels, as compared to the effect on serum concentration of insulin.
  3. To establish the effects of STZ-induced diabetes on brain glucose levels, as compared to the effect on serum glucose concentrations.
  4. To discover if there is a correlation between insulin levels, brain glucose and ATP: ADP ratio, as a potential basis for the earlier observed effect of exocytotic-mode switching in STZ-induced diabetes.
  5. To study the effects of adding insulin directly to diabetic synaptosomes, on the mode of exocytosis.
  6. To look at insulin receptors and insulin-signalling pathways in STZ-induced diabetic rats.
  7. To look at behavioural changes of STZ-induced diabetic rats, when compared to controls, and to see if this has any correlation with the switch in mode of exocytosis.
  8. To study the effects of corticosterone on control rats to see if this induces the same phenotype (re exocytotic mode & behaviour) as the STZ-induced diabetic rats.

Project lead: Dr Anthony Christopher Ashton
Project staff: Dr Anthony Christopher Ashton

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2012 - present